Nature Communications (May 2019)

Hydro-Seq enables contamination-free high-throughput single-cell RNA-sequencing for circulating tumor cells

  • Yu-Heng Cheng,
  • Yu-Chih Chen,
  • Eric Lin,
  • Riley Brien,
  • Seungwon Jung,
  • Yu-Ting Chen,
  • Woncheol Lee,
  • Zhijian Hao,
  • Saswat Sahoo,
  • Hyun Min Kang,
  • Jason Cong,
  • Monika Burness,
  • Sunitha Nagrath,
  • Max S. Wicha,
  • Euisik Yoon

DOI
https://doi.org/10.1038/s41467-019-10122-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Transcriptome analysis of circulating tumor cells (CTCs) provides insights into monitoring target therapeutics and underlying tumor metastasis. Here the authors present Hydro-Seq, a contamination-free high-throughput hydrodynamic scRNA-seq barcoding technique for rare CTCs.