Cytokeratin-7 signature in the prostate of total and sub-capsular orchidectomized wistar rat following experimentally-induced benign prostatic hyperplasia: a comparative study

Abstract

Background: Evidences to establish superiority between Total orchidectomy (TT orchi) and Sub-capsular orchidectomy (SC orchi) as surgical interventions for prostatic enlargement are scarce. Cytokeratins have high specificity profile as benign and tumor markers. Aim and Objectives: To compare the outcomes of TT orchi and SC orchion Benign Prostatic Hyperplasia (BPH). Material and Methods: Fifteen adults male Wistar rats (140-200 g) were grouped into three (A-C) n=5. Group A is the control while B and C received 10 mg/kg testosterone intramuscularly to induce BPH for 10 days. After that, Group A underwent sham surgery, B, TT orchi and C, SC orchi. Surgeries were performed under ketamine (5 mg/kg) and 2-0 chromic was used. Rats were observed for 14 days post-surgically after which they were sacrificed under anesthesia. Blood samples were collected for biochemical assays. Histological and immunohistochemical analyses were done. Data were analyzed using Oneway ANOVAand Student Newman-Keuls. Results were presented as Mean ± SEM, p £0.05 was significant. Results: SOD and CAT were raised in both TTorchi and SC orchi. Prostate Specific Antigen (PSA) was reduced significantly in SC orchi compared to TT orchi. Cytokeratin7 reactivity was positive in TT orchi but negative in others. Conclusion: SC orchi may be considered over TT orchi as BPH surgical therapy.

Keywords

• total orchidectomy
• sub-capsular orchidectomy
• cytokeratin 7
• benign prostatic hyperplasia