Bio-Protocol
(Jul 2017)
Mapping RNA Sequences that Contact Viral Capsid Proteins in Virions
C. Cheng Kao,
Ella Chuang,
James Ford,
Jie Huang,
Ram Podicheti,
Douglas Rusch
Affiliations
C. Cheng Kao
Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA
Ella Chuang
Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA
James Ford
The Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA
Jie Huang
The Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA
Ram Podicheti
The Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA
Douglas Rusch
The Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA
DOI
https://doi.org/10.21769/BioProtoc.2398
Journal volume & issue
Vol. 7,
no. 14
Abstract
Read online
We have adapted the methodology of CLIP-seq (Crosslinking-Immunoprecipitation and DNA Sequencing) to map the segments of encapsidated RNAs that contact the protein shells of virions. Results from the protocol report on the RNA sequences that contact the viral capsid.
WeChat QR code
Close
