Frontiers in Microbiology (Sep 2017)
Differential Characteristics of Viral siRNAs between Leaves and Roots of Wheat Plants Naturally Infected with Wheat Yellow Mosaic Virus, a Soil-Borne Virus
- Linying Li,
- Linying Li,
- Linying Li,
- Ida Bagus Andika,
- Yu Xu,
- Yu Xu,
- Yan Zhang,
- Yan Zhang,
- Xiangqi Xin,
- Lifeng Hu,
- Lifeng Hu,
- Zongtao Sun,
- Zongtao Sun,
- Gaojie Hong,
- Gaojie Hong,
- Yang Chen,
- Yang Chen,
- Fei Yan,
- Fei Yan,
- Jian Yang,
- Jian Yang,
- Junmin Li,
- Junmin Li,
- Junmin Li,
- Jianping Chen,
- Jianping Chen,
- Jianping Chen
Affiliations
- Linying Li
- College of Plant Protection, Nanjing Agricultural UniversityNanjing, China
- Linying Li
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Linying Li
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Ida Bagus Andika
- Group of Plant-Microbe Interactions, Institute of Plant Science and Resources, Okayama UniversityKurashiki, Japan
- Yu Xu
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Yu Xu
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Yan Zhang
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Yan Zhang
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Xiangqi Xin
- Institute of Plant Protection, Shandong Academy of Agricultural SciencesJinan, China
- Lifeng Hu
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Lifeng Hu
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Zongtao Sun
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Zongtao Sun
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Gaojie Hong
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Gaojie Hong
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Yang Chen
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Yang Chen
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Fei Yan
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Fei Yan
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Jian Yang
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Jian Yang
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Junmin Li
- College of Plant Protection, Nanjing Agricultural UniversityNanjing, China
- Junmin Li
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Junmin Li
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Jianping Chen
- College of Plant Protection, Nanjing Agricultural UniversityNanjing, China
- Jianping Chen
- The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Zhejiang Academy of Agricultural SciencesHangzhou, China
- Jianping Chen
- Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural SciencesHangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2017.01802
- Journal volume & issue
-
Vol. 8
Abstract
RNA silencing is an important innate antiviral defense in plants. Soil-borne plant viruses naturally infect roots via soil-inhabiting vectors, but it is unclear how antiviral RNA silencing responds to virus infection in this particular tissue. In this study, viral small interfering RNA (siRNA) profiles from leaves and roots of wheat plants naturally infected with a soil-borne virus, wheat yellow mosaic virus (WYMV, genus Bymovirus), were analyzed by deep sequencing. WYMV siRNAs were much more abundant in roots than leaves, which was positively correlated with the accumulation of viral RNA. WYMV siRNAs in leaves and roots were predominantly 21- and 22-nt long and equally derived from the positive- and negative-strands of the viral genome. WYMV siRNAs from leaves and roots differed in distribution pattern along the viral genome. Interestingly, compared to siRNAs from leaves (and most other reports), those from roots obviously had a lower A/U bias at the 5′-terminal nucleotide. Moreover, the expression of Dicer-like genes upon WYMV infection were differently regulated between leaves and roots. Our data suggest that RNA silencing in roots may operate differently than in leaves against soil-borne virus invasion.
Keywords
- soil-borne plant viruses
- wheat yellow mosaic virus
- viral small interfering RNA
- antiviral RNA silencing
- deep sequencing
