Laboratory sprayer for dsRNA application: Design and bioassay validation
Triin Kallavus,
Riina Kaasik,
Tõnu Leemet,
Kaarel Soots,
Liina Soonvald,
Silva Sulg,
Eve Veromann
Affiliations
Triin Kallavus
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia; Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium; Corresponding author at: Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia.
Riina Kaasik
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia
Tõnu Leemet
Institute of Forestry and Engineering, Estonian University of Life Sciences, Tartu 51006, Estonia
Kaarel Soots
Institute of Forestry and Engineering, Estonian University of Life Sciences, Tartu 51006, Estonia
Liina Soonvald
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia
Silva Sulg
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia
Eve Veromann
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu 51006, Estonia
The shortage of commercially available and reliable laboratory spraying equipment for testing different preparations can be a major obstacle to achieve field-comparable results in the laboratory conditions.RNA interference is natural biological process which, when used for plant protection, can be designed method combining sustainability and minimal environmental side effects. Spraying of dsRNA is a field-relevant method that should ensure consistency and repeatability if conducted in laboratory.We built a portable spray device for laboratory use and tested its suitability for dsRNA application. For that, we carried out bioassay on three plant species with different leaf surface textures. DsRNA were detected in all samples 3 days post-treatment indicating its suitability for dsRNA delivery.We built a portable spray device for laboratory use and tested its suitability for dsRNA application. For that, we carried out: • Bioassay on three plant species with different leaf surface textures.DsRNA were detected in all samples 3 days post-treatment indicating its suitability for dsRNA delivery.
