Relationships between HBeAg status of patients with chronic hepatitis B and changes in serum TNF-α, viral load and severity of morphological changes in the liver according to non-invasive tests

Abstract

Aim – to investigate the relationship between HBeAg status of patients with chronic hepatitis B and the content of TNF-α in the serum, the level of viral load and the severity of morphological changes in the liver according to non-invasive tests. Material and methods. 70 patients with CHB were examined. Noninvasive methods were used to determine the severity of morphological changes in the liver. The content of HBV-DNA in the blood was determined by PCR, HBeAg, anti-HBe, TNF-α by ELISA. Statistical processing was performed in Statistica 13 for Windows (StatSoft Inc., No. JPZ804I382130ARCN10-J). Results. HBeAg-negative patients (78.6 %) with anti-HBe (89.1 %) predominate among patients with CHB. Lower frequency of seroconversion in patients with stages F 2–4, compared with patients with stages F 0-1 (85.7 % vs. 100 %, P 20000 IU/ml (83.3 % vs. 17.7 %). Viral load in HBeAg-positive patients with activity A 0–1 was the highest (P 20000 IU/ml, compared with HBeAg-negative (P 20000 IU/ml in the presence of A 2–3 than in A 0–1. The highest content of TNF-α is in the serum of HBeAg-positive patients with F 2–4, compared with HBeAg-negative patients and the corresponding liver fibrosis (P < 0.05).

Keywords

• chronic hepatitis b
• hbeag
• cytokines
• liver fibrosis