Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil

Abstract

In Brazil, mineral water is widely consumed and as of yet there have been no studies done in the country that aim to detect enteric viruses in this water source. The aim of this study was to analyze the presence of the human adenovirus (HAdV), the human rotavirus genogroup A (GARV) and the human enterovirus (hEV) in mineral water samples from six different brands that are commercialized in southern Brazil, using molecular techniques and comparing the results with bacterial indicators. Samples of 1.5 L and 500 mL were analyzed for viruses through PCR and total and thermotolerant coliforms. Additionally, heterotrophic bacteria were assayed using a commercial kit. The most prevalent virus was adenovirus (32.5%) followed by rotavirus (25%) and enterovirus (17.5%). Total and thermotolerant coliforms were absent in all samples and only three samples out of the 60 analyzed presented heterotrophic bacteria contamination. We conclude that, following the example taken into consideration regarding the public supply of drinking water, stricter measures for microbiological control should also be applied to mineral water so that this actually becomes a safer alternative.

Keywords

• enteric viruses
• HAdV
• hEV
• GARV
• mineral water