Effects of glyprolines on free-radical oxidation in the brain neocortex of white rats in mild traumatic brain injury

Abstract

The aim of the study was to compare the effect of glyproline peptides RGRGP (Arg-Gly-Arg-Gly-Pro), RGP (Arg-GlyPro), PRPGP (Pro-Arg-Pro-Gly-Pro) and PGPL (Pro-Gly-Pro-Leu) peptide substances at various concentrations on the free radical oxidation intensity of the brain tissues of Wistar males after intraperitoneal administration of peptide solutions after traumatic brain injury.Material and methods. The brain tissue of Wistar males aged 2–3 months (n = 126) were used in the experiment. RGRGP, RGP, PRPGP, and PGPL peptides were provided by Academician N.F. Myasoyedov. Traumatic brain injury (TBI) was modeled by free fall of a load. From the second to the fifth day of the experiment, the animals were injected intraperitoneally with peptides. On the sixth day, the animals were taken out of the experiment. The activity of free radical oxidation was determined in freshly prepared homogenates of sections of the cerebral cortex by chemiluminescence (CL).Results. TBI significantly enhance free-radical oxidation intensity of the neocortex in brain tissue of Wistar rats, and the studied peptides affect it in different ways - from a decrease in CL intensity (the minimum value in TBI + RGP 0.1 group) to its increase (the maximum value in TBI + RGPGP 0.1 group). The effect depends on the dose of glyproline.Conclusions. The results obtained, based on the analysis of the free radical oxidation intensity of tissues, mainly indicate a different degree of correction of tissue homeostasis indicators. It can be assumed that Arg-Pro-Gly peptide can be the basis for the development of new drugs for post-stress rehabilitation after injuries of various levels and genesis.

Keywords

• glyprolines
• regulatory peptides
• free-radical oxidation
• neocortex
• traumatic brain injury