IUCrJ (Nov 2017)
Analysis of XFEL serial diffraction data from individual crystalline fibrils
- David H. Wojtas,
- Kartik Ayyer,
- Mengning Liang,
- Estelle Mossou,
- Filippo Romoli,
- Carolin Seuring,
- Kenneth R. Beyerlein,
- Richard J. Bean,
- Andrew J. Morgan,
- Dominik Oberthuer,
- Holger Fleckenstein,
- Michael Heymann,
- Cornelius Gati,
- Oleksandr Yefanov,
- Miriam Barthelmess,
- Eirini Ornithopoulou,
- Lorenzo Galli,
- P. Lourdu Xavier,
- Wai Li Ling,
- Matthias Frank,
- Chun Hong Yoon,
- Thomas A. White,
- Saša Bajt,
- Anna Mitraki,
- Sebastien Boutet,
- Andrew Aquila,
- Anton Barty,
- V. Trevor Forsyth,
- Henry N. Chapman,
- Rick P. Millane
Affiliations
- David H. Wojtas
- Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, New Zealand
- Kartik Ayyer
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Mengning Liang
- Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA
- Estelle Mossou
- Institut Laue-Langevin, Grenoble, France
- Filippo Romoli
- European Synchrotron Radiation Facility, Grenoble, France
- Carolin Seuring
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Kenneth R. Beyerlein
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Richard J. Bean
- European XFEL GmbH, Hamburg, Germany
- Andrew J. Morgan
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Dominik Oberthuer
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Holger Fleckenstein
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Michael Heymann
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Cornelius Gati
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Oleksandr Yefanov
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Miriam Barthelmess
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Eirini Ornithopoulou
- Department of Materials Science and Technology, University of Crete and IESL/FORTH, Crete, Greece
- Lorenzo Galli
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- P. Lourdu Xavier
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Wai Li Ling
- Université Grenoble Alpes, Grenoble, France
- Matthias Frank
- Lawrence Livermore National Laboratory, Livermore, California, USA
- Chun Hong Yoon
- Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA
- Thomas A. White
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Saša Bajt
- Photon Science, DESY, Hamburg, Germany
- Anna Mitraki
- Department of Materials Science and Technology, University of Crete and IESL/FORTH, Crete, Greece
- Sebastien Boutet
- Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA
- Andrew Aquila
- Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA
- Anton Barty
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- V. Trevor Forsyth
- Institut Laue-Langevin, Grenoble, France
- Henry N. Chapman
- Centre for Free-Electron Laser Science, DESY, Hamburg, Germany
- Rick P. Millane
- Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, New Zealand
- DOI
- https://doi.org/10.1107/S2052252517014324
- Journal volume & issue
-
Vol. 4,
no. 6
pp. 795 – 811
Abstract
Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
Keywords
- serial crystallography
- coherent X-ray diffractive imaging (CXDI)
- single particles
- molecular orientation determination
- crystalline fibrils
- amyloid
