Generation of human induced pluripotent stem cell line (WMUi001-A) from a patient with aortic dissection

Peifeng Jin; Sixian Wang; Xu Jiang; Xiaoqiong Shan; Sheng Jiang; Yingyi Quan; Tianci Zhang; Jiwen Yang; Huang Zhang; Jianshe Ma; Miaowu Dong; Junming Fan; Xiaofang Fan; Yongsheng Gong; Yongyu Wang

doi:10.1016/j.scr.2020.101730

Stem Cell Research (Mar 2020)

Generation of human induced pluripotent stem cell line (WMUi001-A) from a patient with aortic dissection

Peifeng Jin,
Sixian Wang,
Xu Jiang,
Xiaoqiong Shan,
Sheng Jiang,
Yingyi Quan,
Tianci Zhang,
Jiwen Yang,
Huang Zhang,
Jianshe Ma,
Miaowu Dong,
Junming Fan,
Xiaofang Fan,
Yongsheng Gong,
Yongyu Wang

Affiliations

Peifeng Jin: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China
Sixian Wang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Xu Jiang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Xiaoqiong Shan: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Sheng Jiang: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China
Yingyi Quan: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Tianci Zhang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Jiwen Yang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Huang Zhang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Jianshe Ma: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Miaowu Dong: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Junming Fan: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Xiaofang Fan: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Yongsheng Gong: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Yongyu Wang: Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China; Corresponding author.

DOI: https://doi.org/10.1016/j.scr.2020.101730
Journal volume & issue: Vol. 43

Abstract

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A human induced pluripotent stem cell (hiPSC) line (WMUi001-A) was generated from the aortic tissue of a 47-year-old donor with aortic dissection and normal blood pressure. Integration-free episomal vector-mediated reprogramming was used for the generation of this iPSC line. The established iPSC line was found to express pluripotency markers, exhibit a differentiation potential in vitro, as well as display a normal karyotype. We further identified that this iPSC line contained a mutation in collagen type IV (COL4A2, R131M), which may serve as a useful tool for the disease modeling of aortic dissection.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.sciencedirect.com/journal/stem-cell-research

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