In Situ Gene Expression in Native Cryofixed Bone Tissue
Krisztina Nikovics,
Cédric Castellarin,
Xavier Holy,
Marjorie Durand,
Halima Morin,
Abdelhafid Bendahmane,
Anne-Laure Favier
Affiliations
Krisztina Nikovics
Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, France
Cédric Castellarin
Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, France
Xavier Holy
Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, France
Marjorie Durand
Osteo-Articulary Biotherapy Unit, Department of Medical and Surgical Assistance to the Armed Forces, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, France
Halima Morin
National Research Institute for Agriculture, Food and the Environment (INRAE), Institute of Plant Sciences Paris-Saclay (IPS2), University Paris-Saclay, 91400 Orsay, France
Abdelhafid Bendahmane
National Research Institute for Agriculture, Food and the Environment (INRAE), Institute of Plant Sciences Paris-Saclay (IPS2), University Paris-Saclay, 91400 Orsay, France
Anne-Laure Favier
Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, France
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood. Large bone defects can be caused by gunshot injury, trauma, accidents, congenital anomalies and tissue resection due to cancer. Therefore, understanding bone homeostasis and regeneration has considerable clinical and scientific importance in the development of bone therapy. Macrophages are well known innate immune cells secreting different combinations of cytokines and their role in bone regeneration during bone healing is essential. Here, we present a method to identify mRNA transcripts in cryosections of non-decalcified rat bone using in situ hybridization and hybridization chain reaction to explore gene expression in situ for better understanding the gene expression of the bone tissues.
