Nature Communications (Oct 2019)

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

  • Wei He,
  • Liang Zhang,
  • Oscar D. Villarreal,
  • Rongjie Fu,
  • Ella Bedford,
  • Jingzhuang Dou,
  • Anish Y. Patel,
  • Mark T. Bedford,
  • Xiaobing Shi,
  • Taiping Chen,
  • Blaine Bartholomew,
  • Han Xu

DOI
https://doi.org/10.1038/s41467-019-12489-8
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.