Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds
Soumaya Bourgou,
Iness Bettaieb Rebey,
Sofiene Ben Kaab,
Majdi Hammami,
Sarra Dakhlaoui,
Selmi Sawsen,
Kamel Msaada,
Hiroko Isoda,
Riadh Ksouri,
Marie-Laure Fauconnier
Affiliations
Soumaya Bourgou
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Iness Bettaieb Rebey
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Sofiene Ben Kaab
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Majdi Hammami
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Sarra Dakhlaoui
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Selmi Sawsen
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Kamel Msaada
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Hiroko Isoda
Faculty of Life and Environmental Sciences, School of Integrative and Global Majors (SIGMA), and Alliance for Research on Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 3058572, Japan
Riadh Ksouri
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia
Marie-Laure Fauconnier
Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030 Gembloux, Belgium
A comparative study of bioactive lipids extraction from black cumin (Nigella sativa L.) and basil (Ocimum basilicum L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, trans-hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities.
