Whole Genome Sequence of <i>Dermacoccus</i> <i>abyssi </i>MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors
Wael M. Abdel-Mageed,
Bertalan Juhasz,
Burhan Lehri,
Ali S. Alqahtani,
Imen Nouioui,
Dawrin Pech-Puch,
Jioji N. Tabudravu,
Michael Goodfellow,
Jaime Rodríguez,
Marcel Jaspars,
Andrey V. Karlyshev
Affiliations
Wael M. Abdel-Mageed
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Bertalan Juhasz
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen AB24 3UE, UK
Burhan Lehri
School of Life Sciences Pharmacy and Chemistry, Faculty of Science, Engineering and Computing, Kingston University, Penrhyn Road, Kingston upon Thames KT1 2EE, UK
Ali S. Alqahtani
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Imen Nouioui
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK
Dawrin Pech-Puch
Centro de Investigacións Científicas Avanzadas (CICA) e Departamento de Química, Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain
Jioji N. Tabudravu
School of Forensic and Applied Sciences, Faculty of Science and Technology, University of Central Lancashire, Preston PR1 2HE, UK.
Michael Goodfellow
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK
Jaime Rodríguez
Centro de Investigacións Científicas Avanzadas (CICA) e Departamento de Química, Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain
Marcel Jaspars
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen AB24 3UE, UK
Andrey V. Karlyshev
School of Life Sciences Pharmacy and Chemistry, Faculty of Science, Engineering and Computing, Kingston University, Penrhyn Road, Kingston upon Thames KT1 2EE, UK
Dermacoccus abyssi strain MT1.1T is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.
