PLoS Neglected Tropical Diseases (Jan 2025)

Vaccination of mice with Trichinella spiralis C-type lectin elicited the protective immunity and enhanced gut epithelial barrier function.

  • Bo Ning Wang,
  • Xin Zhuo Zhang,
  • Jin Yi Wu,
  • Zhao Yu Zhang,
  • Pei Kun Cong,
  • Wen Wen Zheng,
  • Shao Rong Long,
  • Ruo Dan Liu,
  • Jing Cui,
  • Zhong Quan Wang

DOI
https://doi.org/10.1371/journal.pntd.0012825
Journal volume & issue
Vol. 19, no. 1
p. e0012825

Abstract

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BackgroundC-type lectin (CTL) plays an important act in parasite adhesion, host's cell invasion and immune escape. Our previous studies showed that recombinant Trichinella spiralis C-type lectin (rTsCTL) mediated larval invasion of enteral mucosal epithelium. The aim of this study was to investigate protective immunity produced by vaccination with rTsCTL and its effect on gut epithelial barrier function in a mouse model.Methodology/principal findingThe ELISA results showed that subcutaneous vaccination of mice with rTsCTL elicited a systemic humoral response (high levels of serum IgG, IgG1/IgG2a and IgA) and significant gut mucosal sIgA responses. The levels of Th1/Th2 cytokines (IFN-γ/IL-4) secreted from spleen, mesenteric lymph nodes and Peyer's patches were distinctly increased at 6 weeks following vaccination (P ConclusionsVaccination of mice with rTsCTL elicited an obvious protective immunity against larval challenge, impeded larval invasion of gut mucosa, enhanced gut epithelial integrity and barrier function, reduced worm burdens; it also alleviated gut and muscle inflammation. TsCTL might be a novel candidate target molecule for anti-Trichinella vaccines.