Molecules (Apr 2024)

Phenylspirodrimane with Moderate Reversal Effect of Multidrug Resistance Isolated from the Deep-Sea Fungus <i>Stachybotrys</i> sp. 3A00409

  • Xinhua Ma,
  • Min Wu,
  • Zhenwei Chen,
  • Fan Cao,
  • Tianhua Zhong,
  • Zhuhua Luo,
  • Zongze Shao,
  • Yonghong Zhang,
  • Limin Chen,
  • Zhiqiang Zhang

DOI
https://doi.org/10.3390/molecules29071685
Journal volume & issue
Vol. 29, no. 7
p. 1685

Abstract

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Two new phenylspirodrimanes, stachybotrins K and L (1 and 2), together with eight known analogues (3–10), were isolated from deep-sea-derived Stachybotrys sp. MCCC 3A00409. Their structures were determined by extensive NMR data and mass spectroscopic analysis. Absolute configurations of new compounds were determined through a comparison of their circular dichroism (CD) spectra with other reported compounds. The possible reversal effects of all compounds were assayed in the resistant cancer cell lines. Stachybotrysin B (8) can reverse multidrug resistance (MDR) in ABCB1-overexpression cells (KBv200, Hela/VCR) at the non-cytotoxic concentration. Doxorubicin accumulation assay and molecular-docking analysis reveal that the mechanism of its reversal MDR effect may be related to the increase in the intracellular concentration of substrate anticancer drugs.

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