Underwater light source changes nitrogen and phosphorus removal pathways by Vallisneria spinulosa Yan growth system

Abstract

Abstract Vallisneria spinulosa Yan (V.spinulosa Yan) with good ability of water purification is often used for ecological restoration of polluted water bodies. However, it is difficult to survive in turbid water bodies due to the low lighting condition. This study explored the feasibility of introducing artificial underwater light source into water bodies with high turbidity to strengthen the water restoration by V.spinulosa Yan. Addition of underwater light source promoted the clonal reproduction ability of V.spinulosa Yan, thus enhancing the removal loads of total nitrogen (TN), total phosphorus (TP), and nitrate nitrogen ( $${\mathrm{NO}}^{-}_{3}{\mbox{-}}{\mathrm{N}}$$ NO 3 − - N ) by 1.60–3.43 × 10−2, 1.49–3.49 × 10−3, and 0.80–2.06 × 10−2 g m−2 d−1, respectively. Underwater light source significantly reduced the abundance of microbial community on V.spinulosa Yan leaves, as well as most nitrifying bacteria (Nitrosomonadaceae) and denitrifying bacteria (Nitrospira, Comamonadaceae, and Rhodocyclaceae) in the system. But the attachment of some Cyanophyta (Chloroplast and Cyanobacteria) and Photosynthetic bacteria (Rhodobacter) onto the leaves and the growth of Methyloligellaceae in water and sediments were promoted. Nitrogen and phosphorus removal by the growth system of V.spinulosa Yan without underwater light source mainly depended on the biological processes by functional bacteria, while the absorption and co-assimilation effect of V.spinulosa Yan with underwater light source.