不同输入电压等离子体诱变对双对栅藻生长、光合性能及油脂积累的影响Effect of plasma mutation under different input voltages on the growth, photosynthetic performance and lipid accumulation of Scenedesmus bijuba

Abstract

在不同输入电压条件下产生等离子体对双对栅藻进行诱变，并在140 V条件下筛选出3株优势突变藻株（T-2,T-5,T-6）。通过测定培养期间突变株与原始株的生物量、光合性能参数、油脂积累等指标，探究等离子体诱变条件对双对栅藻油脂积累的影响。结果表明：最佳突变株T-5培养35 d的生物量、甘油三酯产量、总脂含量、总脂产率分别为764.1 mg/L、263.0 mg/L、38.2%、8.3 mg/(L·d)，分别较原始株提高了13.6%、58.9%、47.5%、66.0%；T-5突变株的叶绿素a和类胡萝卜素含量增加，使突变株保持良好的光合作用；但T-5突变株的蛋白质含量较原始株下降了24.4%，说明突变株在培养后期以消耗蛋白质为代价以促进油脂的积累。 Scenedesmus bijuba was mutated by plasma under different input voltage conditions, three dominant mutant strains (T-2, T-5, T-6)were screened out at 140 V. By measuring the biomass, photosynthetic performance parameters, lipid accumulation and other indicators of the mutant strain and the original strain during the cultivation period, the effect of plasma mutation conditions on the lipid accumulation of Scenedesmus bijuba was explored. The results showed that the biomass, triglyceride yield, total lipid content, and total lipid productivity of the best mutant strain T-5 cultivated for 35 d were 764.1 mg/L, 263.0 mg/L, 38.2%, 8.3 mg/(L·d), respectively, which increased by 13.6%, 58.9%, 47.5%, 66.0% compared with the original strain. The contents of chlorophyll a and carotenoids of mutant strain T-5 increased, which could maintain good photosynthesis. On the contrary, the protein content of mutant strain T-5 lowered by 24.4% than that of the original strain, indicating that the accumulation of lipid in the mutant strain was promoted at the expense of protein consumption in the later stage of cultivation.

Keywords

• 双对栅藻；等离子体诱变；生长；光合性能；油脂积累；总脂产率
• scenedesmus bijuba; plasma mutagenesis; growth; photosynthetic performance; lipid accumulation; total lipid productivity