BET inhibition disrupts transcription but retains enhancer-promoter contact

Nicholas T. Crump; Erica Ballabio; Laura Godfrey; Ross Thorne; Emmanouela Repapi; Jon Kerry; Marta Tapia; Peng Hua; Christoffer Lagerholm; Panagis Filippakopoulos; James O. J. Davies; Thomas A. Milne

doi:10.1038/s41467-020-20400-z

Nature Communications (Jan 2021)

BET inhibition disrupts transcription but retains enhancer-promoter contact

Nicholas T. Crump,
Erica Ballabio,
Laura Godfrey,
Ross Thorne,
Emmanouela Repapi,
Jon Kerry,
Marta Tapia,
Peng Hua,
Christoffer Lagerholm,
Panagis Filippakopoulos,
James O. J. Davies,
Thomas A. Milne

Affiliations

Nicholas T. Crump: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Erica Ballabio: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Laura Godfrey: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Ross Thorne: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Emmanouela Repapi: MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Jon Kerry: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Marta Tapia: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford
Peng Hua: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Christoffer Lagerholm: Wolfson Imaging Centre Oxford, MRC Weatherall Institute of Molecular Medicine, University of Oxford
Panagis Filippakopoulos: Structural Genomics Consortium, Nuffield Department of Clinical Medicine, University of Oxford
James O. J. Davies: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Thomas A. Milne: MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, NIHR Oxford Biomedical Research Centre Haematology Theme, Radcliffe Department of Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-020-20400-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The role of BRD4 and Mediator in regulating enhancer-promoter interactions is poorly understood. Here the authors find that treatment with BET inhibitors or pharmacological degradation of BRD4 disrupts transcription while having very little effect on enhancer-promoter interactions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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