The heading-date gene Ghd7 inhibits seed germination by modulating the balance between abscisic acid and gibberellins

Abstract

Seed dormancy of cultivated rice was largely weakened during the progress of domestication. Correct timing and uniformity of seed germination are important for rapid seedling establishment and high-yield production. In the present study, we found that the heading-date gene Ghd7 acted as a negative regulator of germination. A mutant of ghd7 showed low sensitivity to exogenous ABA treatment during seed germination. Further investigation revealed reduced accumulation of ABA in mature ghd7 seeds as a consequence of dampened expression of OsNCED genes. Moreover, elevated GA3 level was detected in seeds of ghd7 mutant during imbibition course, which was attributed to the induction of genes responsible for the synthesis pathways of bioactive GAs. Thus, Ghd7 inhibits seed germination by increasing the ABA/GA3 ratio. Besides revealing pleiotropic effects of Ghd7, our results indicate its role in linking seed germination to growth-phase transition in rice, which would enrich the theoretical basis for future breeding practices.

Keywords

• Ghd7
• Seed germination
• Abscisic acid
• Gibberellins
• ABA/GA3 ratio