Biosensors (Sep 2021)

An Optical Smartphone-Based Inspection Platform for Identification of Diseased Orchids

  • Kuan-Chieh Lee,
  • Yen-Hsiang Wang,
  • Wen-Chun Wei,
  • Ming-Hsien Chiang,
  • Ting-En Dai,
  • Chung-Cheng Pan,
  • Ting-Yuan Chen,
  • Shi-Kai Luo,
  • Po-Kuan Li,
  • Ju-Kai Chen,
  • Shien-Kuei Liaw,
  • Choa-Feng Lin,
  • Chin-Cheng Wu,
  • Jen-Jie Chieh

DOI
https://doi.org/10.3390/bios11100363
Journal volume & issue
Vol. 11, no. 10
p. 363

Abstract

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Infections of orchids by the Odontoglossum ringspot virus or Cymbidium mosaic virus cause orchid disfiguration and are a substantial source of economic loss for orchid farms. Although immunoassays can identify these infections, immunoassays are expensive, time consuming, and labor consuming and limited to sampling-based testing methods. This study proposes a noncontact inspection platform that uses a spectrometer and Android smartphone. When orchid leaves are illuminated with a handheld optical probe, the Android app based on the Internet of Things and artificial intelligence can display the measured florescence spectrum and determine the infection status within 3 s by using an algorithm hosted on a remote server. The algorithm was trained on optical data and the results of polymerase chain reaction assays. The testing accuracy of the algorithm was 89%. The area under the receiver operating characteristic curve was 91%; thus, the platform with the algorithm was accurate and convenient for infection screening in orchids.

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