Molecular Cancer (Jun 2022)
Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge
- Junfeng Jiang,
- Tao Zeng,
- Li Zhang,
- Xingfei Fan,
- Qishu Jin,
- Haitao Ni,
- Yusheng Ye,
- Lipeng Cheng,
- Li Li,
- Liujun Wang,
- Sha Xu,
- Yu Yang,
- Juan Gu,
- Bing Guo,
- Lei Wang,
- Xin Li,
- Yingyi Qin,
- Jiaxi Li,
- Jinjiang Wang,
- Xi Chen,
- Minjuan Wu,
- Qi-long Ying,
- Xingjun Qin,
- Yefei Wang,
- Yue Wang
Affiliations
- Junfeng Jiang
- Histology and Embryology Department, Naval Medical University
- Tao Zeng
- Histology and Embryology Department, Naval Medical University
- Li Zhang
- Department of Pathology, Faculty of Medical Imaging, Naval Medical University
- Xingfei Fan
- Histology and Embryology Department, Naval Medical University
- Qishu Jin
- Department of Histology and Embryology, Harbin Medical University
- Haitao Ni
- Histology and Embryology Department, Naval Medical University
- Yusheng Ye
- Histology and Embryology Department, Naval Medical University
- Lipeng Cheng
- Histology and Embryology Department, Naval Medical University
- Li Li
- Histology and Embryology Department, Naval Medical University
- Liujun Wang
- Histology and Embryology Department, Naval Medical University
- Sha Xu
- Histology and Embryology Department, Naval Medical University
- Yu Yang
- Histology and Embryology Department, Naval Medical University
- Juan Gu
- Histology and Embryology Department, Naval Medical University
- Bing Guo
- Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
- Lei Wang
- Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
- Xin Li
- Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
- Yingyi Qin
- Department of health statistics, Naval Medical University
- Jiaxi Li
- Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Jinjiang Wang
- Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine
- Xi Chen
- Department of Cell and Neurobiology, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California
- Minjuan Wu
- Histology and Embryology Department, Naval Medical University
- Qi-long Ying
- Department of Cell and Neurobiology, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California
- Xingjun Qin
- Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
- Yefei Wang
- Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
- Yue Wang
- Histology and Embryology Department, Naval Medical University
- DOI
- https://doi.org/10.1186/s12943-022-01604-x
- Journal volume & issue
-
Vol. 21,
no. 1
pp. 1 – 8
Abstract
Highlights Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety.
Keywords
