Experimental Liver Peroxidation Against the Background of Limb Ischemia - Reperfusion Injury – Is There a Pathogenic Difference Between Its Modifications?

Abstract

Introduction: Use of the haemostatic tourniquet is an important step in providing first aid in open bleeding injuries. Tourniquet pressure on the extremity triggers local lipid peroxidation. However, the systemic influence of tourniquet thereof has not been fully studied. Aim: This study aimed to evaluate the changes that occur against the background of ischemia-reperfusion syndrome (IRS) and modifications of trauma in the main gland for detoxification – the liver. Material and Methods: In order to estimate the liver condition under the effects of a haemostatic tourniquet, animals were divided into five experimental groups, where modifications of hypoxia were performed as a result of bleeding, IRS and trauma due to mechanical fracture of the thigh. Results: Biochemical study of the liver has shown that each type of such interventions caused the activation of lipid peroxidation in this organ. The highest increase of the malonic dialdehyde rate was observed in response to haemostatic tourniquet combined with blood loss. Additionally, its content was higher in the group combining mechanical trauma and the tourniquet compared to isolated trauma. Conclusion: All types of interventions caused hypoxia – as a result of isolated bleeding and cessation of blood flow due to the tourniquet. However, the release of overconcentration of toxic derivatives of rhabdomyolysis, which entered the blood stream after limb release, activated the pathological mechanisms of IRS, which included intensified lipid peroxidation in the liver.

Keywords

• ischemia-reperfusion syndrome
• liver
• blood loss
• tourniquet
• lipid peroxidation