STUDY ON THE SEPARATION THROUGH THIN LAYER CHROMATOGRAPHY OF THE CHOLESTEROL IN THE BUFFALO MILK ORIGINATING IN THE MESENDORF FARM

Abstract

Within the present study, we conducted a thin layer chromatographic separation of neuter fats in buffalo milk, following sodium metoxyde saponification and the exposition to λ=366 nm. The extraction of free and esterized milk cholesterol was conducted according to the Folch et al. (1957) method. The free and esterized cholesterol, as well as triglycerids appear as brown spots (iodine complexes with the double connections between the compounds under anlysis). Qualitative identification was conducted through the comparison of Rfs for free and esterized cholesterol, in the samples analyzed with existing standards. The semi-quantitative anlysis was conducted through a visual comparison of the intensity encountered in free cholesterol spots within samples under anlysis and the standard of different concentrations applied on the same board. Buffalo milk samples originated in the Mesendorf Farm, considered and certified as an ecologic farm.

Keywords

• cholesterol
• milk
• buffalo