Development of a Polyphenol Oxidase Biosensor from Jenipapo Fruit Extract (Genipa americana L.) and Determination of Phenolic Compounds in Textile Industrial Effluents
Rafael Souza Antunes,
Denes Ferraz,
Luane Ferreira Garcia,
Douglas Vieira Thomaz,
Rafael Luque,
Germán Sanz Lobón,
Eric de Souza Gil,
Flávio Marques Lopes
Affiliations
Rafael Souza Antunes
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), Rua 221 Esquina com a 5ª Avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
Denes Ferraz
Campus Henrique Santilo, BR-153, 3105, Universidade Estadual de Goiás, Fazenda Barreiro do Meio, Anápolis-GO 75132-903, Brazil
Luane Ferreira Garcia
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), Rua 221 Esquina com a 5ª Avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
Douglas Vieira Thomaz
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), Rua 221 Esquina com a 5ª Avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
Rafael Luque
Departamento de Química Orgánica, Universidad de Cordoba, 14014 Cordoba, Spain
Germán Sanz Lobón
Departamento de Química Orgánica, Universidad de Cordoba, 14014 Cordoba, Spain
Eric de Souza Gil
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), Rua 221 Esquina com a 5ª Avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
Flávio Marques Lopes
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), Rua 221 Esquina com a 5ª Avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
In this work, an innovative polyphenol oxidase biosensor was developed from Jenipapo (Genipa americana L.) fruit and used to assess phenolic compounds in industrial effluent samples obtained from a textile industry located in Jaraguá-GO, Brasil. The biosensor was prepared and optimized according to: the proportion of crude vegetal extract, pH and overall voltammetric parameters for differential pulse voltammetry. The calibration curve presented a linear interval from 10 to 310 µM (r2 = 0.9982) and a limit of detection of 7 µM. Biosensor stability was evaluated throughout 15 days, and it exhibited 88.22% of the initial response. The amount of catechol standard recovered post analysis varied between 87.50% and 96.00%. Moreover, the biosensor was able to detect phenolic compounds in a real sample, and the results were in accordance with standard spectrophotometric assays. Therefore, the innovatively-designed biosensor hereby proposed is a promising tool for phenolic compound detection and quantification when environmental contaminants are concerned.
