Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

Abstract

Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow to improve serological diagnostics of lumpy skin disease (LSD).

Keywords

• antibodies
• elisa
• lumpy skin disease virus
• virus neutralization test
• specificity
• sensitivity