Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB

Lee A. Rettberg; Jarett Wilcoxen; Andrew J. Jasniewski; Chi Chung Lee; Kazuki Tanifuji; Yilin Hu; R. David Britt; Markus W. Ribbe

doi:10.1038/s41467-020-15627-9

Nature Communications (Apr 2020)

Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB

Lee A. Rettberg,
Jarett Wilcoxen,
Andrew J. Jasniewski,
Chi Chung Lee,
Kazuki Tanifuji,
Yilin Hu,
R. David Britt,
Markus W. Ribbe

Affiliations

Lee A. Rettberg: Department of Molecular Biology and Biochemistry, University of California
Jarett Wilcoxen: Department of Chemistry, University of California
Andrew J. Jasniewski: Department of Molecular Biology and Biochemistry, University of California
Chi Chung Lee: Department of Molecular Biology and Biochemistry, University of California
Kazuki Tanifuji: Department of Molecular Biology and Biochemistry, University of California
Yilin Hu: Department of Molecular Biology and Biochemistry, University of California
R. David Britt: Department of Chemistry, University of California
Markus W. Ribbe: Department of Molecular Biology and Biochemistry, University of California

DOI: https://doi.org/10.1038/s41467-020-15627-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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NifB is a radical SAM enzyme involved in the biosynthesis of the Mo-nitrogenase cofactor, which is responsible for the ambient conversion of N2 to NH3. Here, the authors identify and uncover the function of a His43 residue as an essential nitrogen ligand of NifB in cofactor biosynthesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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