Gill transcriptomes analysis of Takifugu obscurus, Takifugu rubripes and their hybrid offspring in freshwater and seawater

Yushun Tian; Chenqi Wang; Yaohui Wang; Yuyu Xiong; Ying Liu; Hongwei Yan; Aijun Wu; Rui Gao; Meiyuan Li; Liu Wang; Jinfeng Chen; Mingtao Hu; Jianhua Ye; Qi Liu; Xiuli Wang

Aquaculture Reports (Aug 2024)

Gill transcriptomes analysis of Takifugu obscurus, Takifugu rubripes and their hybrid offspring in freshwater and seawater

Yushun Tian,
Chenqi Wang,
Yaohui Wang,
Yuyu Xiong,
Ying Liu,
Hongwei Yan,
Aijun Wu,
Rui Gao,
Meiyuan Li,
Liu Wang,
Jinfeng Chen,
Mingtao Hu,
Jianhua Ye,
Qi Liu,
Xiuli Wang

Affiliations

Yushun Tian: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China
Chenqi Wang: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China
Yaohui Wang: Jiangsu Zhongyang Group Company Limited, Haian, Jiangsu 226600, China
Yuyu Xiong: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Ying Liu: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China
Hongwei Yan: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China
Aijun Wu: Jiangsu Zhongyang Group Company Limited, Haian, Jiangsu 226600, China
Rui Gao: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Meiyuan Li: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Liu Wang: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Jinfeng Chen: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Mingtao Hu: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China
Jianhua Ye: Jiangsu Zhongyang Group Company Limited, Haian, Jiangsu 226600, China
Qi Liu: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; College of Marine Science and Environment Engineering, Dalian Ocean University, Dalian 116023, China; Corresponding author at: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China.
Xiuli Wang: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, China; Corresponding author at: Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China.

Journal volume & issue: Vol. 37
p. 102208

Abstract

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Osmoregulation is essential for aquatic organisms to balance their internal fluids and ions and adapt to environmental changes. The gills are a crucial organ for maintaining osmotic balance in fish by regulating the uptake and excretion of ions. Changes in the salinity of the aquatic environment can lead to alterations in the expression of genes within gill cells, which helps to maintain the internal salinity balance and allows the fish to adapt to different salinity levels. In this study, we used transcriptomic data of the gill from the euryhaline Takifugu obscurus and the stenohaline Takifugu rubripes, as well as their hybrid offspring (Tor1 and Tor2), to investigate how the gill contributes to osmoregulation in response to freshwater and seawater. Our study revealed 8 commonly differentially expressed genes among these four fish species. Analysis of the T. obscurus transcriptome data has demonstrated that SLC1a5, a member of the solute carrier (SLC) family, and Gper1, a G-protein coupled estrogen receptor 1-like, are enriched in many biological processes and might be important for its osmoregulation in freshwater. Furthermore, PDE8B, Lrrc9 and Cplx4 might be important osmoregulatory genes that are inherited by offspring in T. obscurus. Moreover, upon encountering changes in water salinity, T. rubripes and the hybrid offspring Tor1 reveal a marked concentration of differentially expressed genes in metabolic pathways within their gills, whereas T. obscurus and the hybrid offspring Tor2 show a more even distribution of DEGs across various signaling pathways. Finally, the Pearson correlation analysis revealed that the T. rubripes displayed a significant correlation with Tor1, while the T. obscurus was significantly related to Tor2. Overall, this study provides a theoretical foundation for understanding the salinity adaptation mechanisms in hybrid offspring of Takifugu, and holds significant implications for guiding the breeding of economically valuable Takifugu strains with improved traits.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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