Nature Communications (May 2021)

Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

  • Sota Takanezawa,
  • Takashi Saitou,
  • Takeshi Imamura

DOI
https://doi.org/10.1038/s41467-021-23249-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

Read online

Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.