Selection of Pisum sativum L. distinguished from callus culture stems and leaves resistant to Fusarium solani

Abstract

Abstract Callus was stimulated from leaves and stems explants of Pea seedlings on MS media supplemented with growth regulators. Medium containing 1.5 mg/L BA and 2.0 mg/L NAA was the most favorable for callus induction from leaves. Whereas MS medium containing 2.0 mg/L BA and 4.0 mg/L IAA was the best for stem callus. Selection of resistant callus to the virulent strains of Fusarium solani was examined from culture medium that contain the concentration 2.5, 5.0 7.5, 10.0, 12.5. 15.5. 17.5 and 20.0 % of filtrates. The concentration which allowed selection were 10.0 and 12.5 %. Regeneration of pea plants from callus of leaves and stems were resisted the toxic effects of filtrate. In spite of reduction of the regeneration ability of the resistant callus up to 45 and 26% derived from leave and steam respectively compared with control. The chromosome number assay showed no difference between resistant and control plants, Pea plant regenerated from tolerate callus exhibited clear resistance when inoculated with virulent fungi.

Keywords

• selection of pisum sativum l
• fusarium solani
• callus culture