Two New Compounds from the Endophytic Fungi of <i>Dryopteris crassirhizoma</i> and Their Antimicrobial Activities
Ping Hai,
Yuan Gao,
Lian Yang,
Nie Chen,
Haiyan Jia,
Mengdie Wang,
Huan Li,
Wenli Jiang,
Jian Yang,
Rongtao Li
Affiliations
Ping Hai
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Yuan Gao
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Lian Yang
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Nie Chen
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Haiyan Jia
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Mengdie Wang
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Huan Li
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Wenli Jiang
Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China
Jian Yang
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medical, China Academy of Chinese Medical Sciences, Beijing 100010, China
Rongtao Li
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Two endophytic fungi Trichoderma afroharzianum (HP-3) and Alternaria alstroemeriae (HP-7) were isolated and purified from the fresh root of Dryopteris crassirhizoma. Chemical investigation of the two fungi resulted in the isolation of two new phenols 2,4-dihydroxy-3-farnesyl-5-methoxy benzoic acid (1) and 2-hydroxyphenethyl 2-phenylacetate (2), together with 22 known compounds. Their structures were elucidated by NMR, UV, IR, HRESIMS, and comparison to the literature data. Compounds 15 and 16 showed significant antibacterial activity against Micrococcus lysodeikticus with MIC value of 6.25 μg/mL, while 8 and 14 displayed moderate inhibitory activities against several plant pathogenic fungi and clinically important bacterial strains. This is the first study to report the isolation, identification, and antimicrobial properties of metabolites from endophytic fungi of D. crassirhizoma. Our findings may provide lead compounds for the development of new antibacterial agents.
