Plasmodium falciparum molecular surveillance to inform the Mozambican National Malaria Control Programme strategy: protocol
Robert Verity,
Patrick Walker,
Alfredo Mayor,
Bryan Greenhouse,
Baltazar Candrinho,
Clemente da Silva,
Eduard Rovira-Vallbona,
Arlindo Chidimatembue,
Paulo Arnaldo,
Simone Boene,
Gloria Matambisso,
Arnau Pujol,
Kiba Comiche,
Antoni Sánchez,
Andrés Aranda-Díaz,
Cristina Ariani,
Henriques Mbeve,
Nelo Ndimande,
Dário Tembisse,
Shazia Ruybal-Pesántez,
Bernardete Rafael
Affiliations
Robert Verity
Medical Research Council Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK
Patrick Walker
6 MRC Centre for Global Infectious Disease Analysis, Imperial College London, London, UK
Alfredo Mayor
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Bryan Greenhouse
3 EPPIcenter Research Program, Division of HIV, Infectious Diseases, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA
Baltazar Candrinho
7 National Malaria Control Program, Ministry of Health, Maputo, Mozambique
Clemente da Silva
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Eduard Rovira-Vallbona
2 ISGlobal, Barcelona, Spain
Arlindo Chidimatembue
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Paulo Arnaldo
4 Instituto Nacional de Saúde, Maputo, Mozambique
Simone Boene
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Gloria Matambisso
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Arnau Pujol
2 ISGlobal, Barcelona, Spain
Kiba Comiche
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Antoni Sánchez
2 ISGlobal, Barcelona, Spain
Andrés Aranda-Díaz
3 EPPIcenter Research Program, Division of HIV, Infectious Diseases, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA
Cristina Ariani
5 Wellcome Sanger Institute, Hinxton, Cambridge, UK
Henriques Mbeve
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Nelo Ndimande
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Dário Tembisse
1 Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique
Shazia Ruybal-Pesántez
6 MRC Centre for Global Infectious Disease Analysis, Imperial College London, London, UK
Bernardete Rafael
7 National Malaria Control Program, Ministry of Health, Maputo, Mozambique
Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise the effectiveness of antimalarial interventions. This study aims to streamline surveillance activities to inform the new strategic plan of the Mozambican National Malaria Control Programme (2023–2030) for malaria control and elimination.Methods and analyses This prospective genomic surveillance study aims to generate Plasmodium falciparum genetic data to monitor diagnostic failures due to pfhrp2/3 deletions and molecular markers of antimalarial drug resistance, to characterise transmission sources and to inform the implementation of new antimalarial approaches to be introduced in Mozambique (chemoprevention and child malaria vaccination). The study, to be conducted between 2024 and 2026, will use three sampling schemes: a multicluster probabilistic health facility survey in the 10 provinces of the country to detect pfhrp2/3 deletions and markers of antimalarial drug resistance; dense sampling of all clinical cases in representative districts in the south targeted for elimination to characterise malaria importation and identify sources of transmission; and testing of pregnant women for malaria at their first antenatal care visit to assess malaria burden and molecular trends. Using a multiplex amplicon-based sequencing approach, the study will target microhaplotypes informative of genomic diversity and relatedness, as well as key drug resistance-associated genes, pfhrp2/3 deletion and malaria vaccine targets. Key genomic information will be visualised in a dashboard integrated into the District Health Information System V.2-based Malaria Information Storage System for programmatic use.Ethics and dissemination The protocol was reviewed and approved by the national ethics committee of Mozambique (Comité Nacional de Bioética para Saúde, Ref: 680/CNBS/23). Project results will be presented to all stakeholders using study-specific brochures and published in open-access journals.Trial registration number NCT06529237.