Optimising genomic approaches for identifying vancomycin-resistant Enterococcus faecium transmission in healthcare settings

Charlie Higgs; Norelle L. Sherry; Torsten Seemann; Kristy Horan; Hasini Walpola; Paul Kinsella; Katherine Bond; Deborah A. Williamson; Caroline Marshall; Jason C. Kwong; M. Lindsay Grayson; Timothy P. Stinear; Claire L. Gorrie; Benjamin P. Howden

doi:10.1038/s41467-022-28156-4

Nature Communications (Jan 2022)

Optimising genomic approaches for identifying vancomycin-resistant Enterococcus faecium transmission in healthcare settings

Charlie Higgs,
Norelle L. Sherry,
Torsten Seemann,
Kristy Horan,
Hasini Walpola,
Paul Kinsella,
Katherine Bond,
Deborah A. Williamson,
Caroline Marshall,
Jason C. Kwong,
M. Lindsay Grayson,
Timothy P. Stinear,
Claire L. Gorrie,
Benjamin P. Howden

Affiliations

Charlie Higgs: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Norelle L. Sherry: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Torsten Seemann: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Kristy Horan: Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Hasini Walpola: Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Paul Kinsella: Department of Microbiology, Royal Melbourne Hospital
Katherine Bond: Department of Microbiology, Royal Melbourne Hospital
Deborah A. Williamson: Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Caroline Marshall: Victorian Infectious Diseases Service, The Peter Doherty Institute for Infection and Immunity
Jason C. Kwong: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
M. Lindsay Grayson: Department of Infectious Diseases, Austin Health
Timothy P. Stinear: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Claire L. Gorrie: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne
Benjamin P. Howden: Department of Microbiology & Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne

DOI: https://doi.org/10.1038/s41467-022-28156-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

Vancomycin-resistant Enterococcus faecium is an important healthcare-associated pathogen and genomic analyses could inform targeted interventions. Here, the authors optimise an analysis pipeline for identification of putative transmission events using core genome multilocus sequence type clustering and split kmer analysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal