The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells

Wenzhen Lin; Li Gao; Wenxin Jiang; Chenguang Niu; Keyong Yuan; Xuchen Hu; Rui Ma; Zhengwei Huang

doi:10.1186/s12903-018-0680-6

BMC Oral Health (Jan 2019)

The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells

Wenzhen Lin,
Li Gao,
Wenxin Jiang,
Chenguang Niu,
Keyong Yuan,
Xuchen Hu,
Rui Ma,
Zhengwei Huang

Affiliations

Wenzhen Lin: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Li Gao: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Wenxin Jiang: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Chenguang Niu: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Keyong Yuan: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Xuchen Hu: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Rui Ma: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
Zhengwei Huang: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine

DOI: https://doi.org/10.1186/s12903-018-0680-6
Journal volume & issue: Vol. 19, no. 1
pp. 1 – 8

Abstract

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Abstract Background Extracellular matrix secretion and odontoblastic differentiation in human dental pulp stem cells (hDPSCs) are the cellular bases for reparative dentinogenesis. Osteomodulin (OMD) is a member of the small leucine-rich proteoglycan family distributed in the extracellular matrix but little is known about its role in osteo/odontogenic differentiation. The objective of this study was to investigate the role of OMD during osteo/odontoblastic differentiation of hDPSCs. Methods hDPSCs were selected using immune-magnetic beads and their capability of multi-differentiation was identified. OMD knockdown was achieved using short hairpin RNA (shRNA) lentivirus and was confirmed by western blot. Gene expression was measured by real-time qPCR and osteo/odontoblastic differentiation of hDPSCs was determined by alizarin red S staining. Results Compared with uninduced cells, the transcription of OMD was up-regulated by 35-fold at the late stage of osteo/odontogenic differentiation. shRNA-mediated gene silencing of OMD decreased the expression of odontoblastic genes, such as alkaline phosphatase (ALP), dentin matrix acidic phosphoprotein 1 (DMP1) and dentin sialophosphoprotein (DSPP). Besides, knockdown of OMD attenuated the mineralized nodules formation induced by osteo/odontogenic medium. Conclusions These results implied that OMD may play a pivotal role in modulating the osteo/odontoblastic differentiation of hDPSCs.

Published in BMC Oral Health

ISSN: 1472-6831 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Medicine: Dentistry
Website: http://bmcoralhealth.biomedcentral.com

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Abstract

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