Nature Communications (Jul 2020)

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

  • Stephane Emond,
  • Maya Petek,
  • Emily J. Kay,
  • Brennen Heames,
  • Sean R. A. Devenish,
  • Nobuhiko Tokuriki,
  • Florian Hollfelder

DOI
https://doi.org/10.1038/s41467-020-17061-3
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 14

Abstract

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Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.