Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycle

Esther Griesbach; Margarita Schlackow; William F. Marzluff; Nick J. Proudfoot

doi:10.1038/s41467-020-20520-6

Nature Communications (Jan 2021)

Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycle

Esther Griesbach,
Margarita Schlackow,
William F. Marzluff,
Nick J. Proudfoot

Affiliations

Esther Griesbach: Sir William Dunn School of Pathology, University of Oxford, South Parks Road
Margarita Schlackow: Sir William Dunn School of Pathology, University of Oxford, South Parks Road
William F. Marzluff: Department of Biochemistry and Biophysics, University of North Carolina
Nick J. Proudfoot: Sir William Dunn School of Pathology, University of Oxford, South Parks Road

DOI: https://doi.org/10.1038/s41467-020-20520-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

Read online

H2A.X histone variant gene encodes poly(A)+ and poly(A)- mRNA isoforms which are differentially expressed depending on cell lines. Here the authors show that upon DNA damage, cells expressing more poly(A)+ isoform require this isoform for de novo H2A.X synthesis while cells with more poly(A)- isoform have sufficient H2A.X present in chromatin.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal