Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

Yi Yang; Haiping Song; Dan He; Shuai Zhang; Shizhong Dai; Shixian Lin; Rong Meng; Chu Wang; Peng R. Chen

doi:10.1038/ncomms12299

Nature Communications (Jul 2016)

Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

Yi Yang,
Haiping Song,
Dan He,
Shuai Zhang,
Shizhong Dai,
Shixian Lin,
Rong Meng,
Chu Wang,
Peng R. Chen

Affiliations

Yi Yang: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Haiping Song: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Dan He: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Shuai Zhang: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Shizhong Dai: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Shixian Lin: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Rong Meng: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Chu Wang: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Peng R. Chen: Department of Chemical Biology, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University

DOI: https://doi.org/10.1038/ncomms12299
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

Mapping protein-protein interaction using crosslinking and mass spectroscopy strategies is hampered by a high rate of false-positive results. Here, the authors develop a genetically encoded photo-affinity probe for accurate identification of protein interaction partners and crosslinking sites.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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