Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase

Abstract

The authors report several high-resolution functional snapshots of type III-E nuclease-protease Craspase complexes, revealing the mechanisms underlying target RNA cleavage and non-self RNA activated protease activities; and highlighting the potentials for the development of RNA-guided nuclease-protease Craspase-based tools for biotechnological applications.