Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from <i>Tetraclinis articulata</i> (Vahl) Mast. Growing in Tunisia
Salma Jlizi,
Aida Lahmar,
Afifa Zardi-Bergaoui,
Roberta Ascrizzi,
Guido Flamini,
Abdel Halim Harrath,
Leila Chekir-Ghedira,
Hichem Ben Jannet
Affiliations
Salma Jlizi
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Medicinal Chemistry and Natural Products Team, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Aida Lahmar
Bioactive Natural Products and Biotechnology Research UnitUR17ES49, Faculty of Dental Medicine of Monastir, University of Monastir, Avicenne Street, 5000 Monastir, Tunisia
Afifa Zardi-Bergaoui
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Medicinal Chemistry and Natural Products Team, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Roberta Ascrizzi
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, Italy
Guido Flamini
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, Italy
Abdel Halim Harrath
Department of Zoology, College of Science, King Saud University, Riyadh 11564, Saudi Arabia
Leila Chekir-Ghedira
Bioactive Natural Products and Biotechnology Research UnitUR17ES49, Faculty of Dental Medicine of Monastir, University of Monastir, Avicenne Street, 5000 Monastir, Tunisia
Hichem Ben Jannet
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Medicinal Chemistry and Natural Products Team, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44.4–70.2%) were detected, mainly consisting of caryophyllene oxide (13.1–26.6%), carotol (9.2–21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2–15.5%) and humulene epoxide II (2.6–7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast cancer cell line, showing IC50 values from 25.7 to 96.5 μg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC50 values lower than 30 μg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata.
