ER stress modulates the immune regulatory ability in gut M2 cells of patients with ulcerative colitis

Baisui Feng; Lingzhi Xu; Shuo Song; Huazhen Liu; Yan Li; Suqin Hu; Qing Shu; Jiangqi Liu; Zhiqiang Liu; Haiqiong Yu; Pingchang Yang

iScience (Apr 2023)

ER stress modulates the immune regulatory ability in gut M2 cells of patients with ulcerative colitis

Baisui Feng,
Lingzhi Xu,
Shuo Song,
Huazhen Liu,
Yan Li,
Suqin Hu,
Qing Shu,
Jiangqi Liu,
Zhiqiang Liu,
Haiqiong Yu,
Pingchang Yang

Affiliations

Baisui Feng: Department of Gastroenterology, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Lingzhi Xu: Department of Immunology, School of Basic Medical Sciences, Weifang Medical University, Weifang, China
Shuo Song: Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Division at Shenzhen University, Shenzhen, China
Huazhen Liu: Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Division at Shenzhen University, Shenzhen, China
Yan Li: Department of Gastroenterology, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Suqin Hu: Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Division at Shenzhen University, Shenzhen, China
Qing Shu: Department of Gastroenterology, Shenzhen Second People’s Hospital, Shenzhen, China
Jiangqi Liu: Department of Allergy, Longgang ENT Hospital, Shenzhen, China
Zhiqiang Liu: Department of Allergy, Longgang ENT Hospital, Shenzhen, China
Haiqiong Yu: Department of Pulmonary and Critical Care Medicine, The Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen518033, China; Corresponding author
Pingchang Yang: Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Division at Shenzhen University, Shenzhen, China; Corresponding author

Journal volume & issue: Vol. 26, no. 4
p. 106498

Abstract

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Summary: This study aims to characterize the impaired immune regulatory function of Mφ obtained from UC patient colon lavage fluid (CLF). Mφs were the largest proportion (21.3 4.0%) of the CLF-derived cellular components. Less abundant and weaker immune suppressive function were observed in M2 Mφs (M2 cells) of the ulcerative colitis (UC) group. High levels of endoplasmic reticulum (ER) stress associated molecules were detected in UC M2 cells. The spliced X box binding protein-1 (XBP1) gene was negatively correlated with programmed death ligand-1 (PD-L1) in UC M2 cells. XBP1 promoted the expression of ring-finger protein 20 (Rnf20) in M2 cells. Rnf20 reduced PD-L1 abundance in UC M2 cells and impaired the immune suppressive ability. Inhibition of Rnf20 restored the immune regulating capacity of M2 cells and suppressed experimental colitis.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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