Genetic diversity of Rhombomys opimus and Meriones meridianus with potential divergence of plague resistance in the Junggar Basin plague focus based on RT1-Db1*exon1
Qiguo Wang,
Wenting Mou,
Tao Luo,
Guliayi Baokaixi,
Yongjun Luo,
Maidina Xiaokaiti,
Xiaowukaiti Saimaiti,
Xinhui Wang,
Junhui Hao,
Youjun Gui,
Xiaojun Wang,
Haiyan Wu,
Xijiang Wang
Affiliations
Qiguo Wang
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Wenting Mou
Microbiological Laboratory, Urumqi Center for Disease Control and Prevention, Urumqi, 830000, China
Tao Luo
Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China; Department of Sterilization and Infection Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China
Guliayi Baokaixi
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Yongjun Luo
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Maidina Xiaokaiti
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Xiaowukaiti Saimaiti
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Xinhui Wang
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China
Junhui Hao
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China
Youjun Gui
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China
Xiaojun Wang
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China
Haiyan Wu
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China; Corresponding author. Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, No. 380, Jianquanyi Road, Urumqi, 830000, China.
Xijiang Wang
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China; Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China; Corresponding author. Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China.
In the Junggar Basin plague focus, Rhombomys opimus and Meriones meridianus live together, and their parasitic fleas have a similar community structure. However, R. opimus has significantly higher positive rates of Yersinia pestis and anti-F1 antibody compared with M. meridianus. In this study, Y. pestis- and antiF1 antibody-negative R. opimus and M. penicilliger were collected in Qitai county, Fukang city and Mulei county of the eastern part of the Junggar Basin. The genomic DNA was extracted from their spleen tissues, and RT1-Db1*exon1 was amplified through PCR procedure and then sequenced. Sequence analysis was performed and molecular diversity parameters were calculated and compared. The results showed that there were significant differences in nucleotide composition, amino acid composition, number and distribution of single nucleotide polymorphism (SNP) sites and number of haplotypes between R. opimus and M. penicilliger. The nucleotide diversity (π) for R. opimus was 0.00420 ± 0.00139, the haplotype diversity (h) was 0.833 ± 0.086, and the average number of nucleotide differences (K) was 2.02564. The π for M. penicilliger was 0.06569 ± 0.02524, and the h was 1.000 ± 0.045, and the K was 10.4444. The fixation index (FST) value between R. opimus and M. penicilliger was 0.9207. Furthermore, the FST value within R. opimus (0.0275) was significantly lower than that within M. penicilliger (0.2106), indicating a greater genetic variation of M. penicilliger compared with R. opimus. In conclusion, the genetic diversity analysis based on RT1-Db1*exon1 showed that M. penicilliger had higher gene polymorphism and greater genetic differentiation compared with R. opimus in the Junggar Basin plague focus, which might be associated with the low infection rate of Y. pestis.
