International Journal of Nanomedicine (Nov 2023)
Bovine Serum Albumin – Hydroxyapatite Nanoflowers as Potential Local Drug Delivery System of Ciprofloxacin
Abstract
Kornelia Bobrowska,1 Kamila Sadowska,1 Krzysztof Stolarczyk,2 Marta Prześniak-Welenc,3 Piotr Golec,4 Renata Bilewicz2 1Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland; 2Faculty of Chemistry, University of Warsaw, Warsaw, Poland; 3Institute of Nanotechnology and Materials Engineering, and Advanced Materials Centre, Gdansk University of Technology, Gdansk, Poland; 4Department of Molecular Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, PolandCorrespondence: Kamila Sadowska, Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ks. Trojdena 4, Warsaw, 02-109, Poland, Tel +48 22 59 25 140, Email [email protected]: Hybrid nanoflowers are structures consisting of organic (enzymes, proteins, nucleic acids) and inorganic components (mostly metal phosphates) with a flower-like hierarchical structure. Novel hybrid nanoflowers based on bovine serum albumin (BSA) and hydroxyapatite (HA) were obtained and characterized. Study on BSA-HA nanoflowers as potential drug delivery system is reported for the first time.Methods: Embedding ciprofloxacin in the structure of hybrid nanoflowers was confirmed by ATR-FTIR and thermogravimetric analysis. The inorganic phase of the nanoflowers was determined by X-ray diffraction. UV‒Vis spectroscopy was used to evaluate the release profiles of ciprofloxacin from nanoflowers in buffer solutions at pH 7.4 and 5. The agar disk diffusion method was used to study the antibacterial activity of the synthesized nanoflowers against Staphylococcus aureus and Pseudomonas aeruginosa.Results: Bovine serum albumin – hydroxyapatite nanoflowers were obtained with diameters of ca. 1– 2 μm. The kinetics of ciprofloxacin release from nanoflowers were described by the Korsmeyer-Peppas model. The antibacterial activity of the synthesized nanoflowers was demonstrated against S. aureus and P. aeruginosa, two main pathogens found in osteomyelitis.Conclusion: The formulated nanoflowers may act as an efficient local antibiotic delivery system. Due to the use of nonhazardous, biodegradable components and benign synthesis, hybrid nanoflowers are very promising drug delivery systems that could be applied in the treatment of skeletal system infections. Keywords: hybrid nanoflowers, organic‒inorganic nanoflowers, Staphylococcus aureus, Pseudomonas aeruginosa, osteomyelitis
