International Journal of Nanomedicine (Jan 2023)

The Combined Effect of Nanobubble-IR783-HPPH-Affibody Complex and Laser on HER2-Positive Breast Cancer

  • Cai W,
  • Lv W,
  • Meng L,
  • Duan Y,
  • Zhang L

Journal volume & issue
Vol. Volume 18
pp. 339 – 351

Abstract

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Wenbin Cai,1,2,* Wei Lv,1,3,* Li Meng,2 Yunyou Duan,1 Li Zhang1 1Department of Ultrasound Medical, Tangdu Hospital, the Fourth Military Medical University, Xi’an, People’s Republic of China; 2Department of Ultrasound Diagnostics, General Hospital of Tibet Military Region, Lhasa, People’s Republic of China; 3Department of Radiology, 305 Hospital of Chinese People’s Liberation Army, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li Zhang, Department of Ultrasound Medical, Tangdu Hospital, 569# Xinsi Road, Baqiao District, Xi’an, People’s Republic of China, Tel +86-29-84777171, Fax +86-29-83510181, Email [email protected]: Nanobubble is an innovative ultrasound contrast agent that triggers the development of targeted imaging of HER2-positive breast cancer by combining with HER2 affibody and IR783. HPPH is a second-generation photosensitiser that is effective in treating tumours. Hence, the nanobubble-IR783-HPPH-affibody (NIHA) complex demonstrates considerable potential in the treatment of HER2-positive breast cancer.Methods: We fabricated the NIHA complex via an advanced thin-film hydration method and detected its characteristics such as particle size, morphology, stability, and cytotoxicity. Moreover, the effect of NIHA complex with laser on HER2-positive breast cancer was confirmed via in vitro and in vivo experiments.Results: The NIHA complex was spheroid, stable and exhibited no cytotoxicity; moreover, its particle size was 524.8 ± 53.3 nm (n = 5). In combination with laser treatment, NIHA complex reduced the cell viability and tumour volume, induced apoptosis of HER2-positive breast cancer cells, and prolonged survival of nude mice.Conclusion: The newly prepared NIHA complex with laser treatment has the potential on treating HER2-positive breast cancer.Keywords: HER2, breast cancer, HPPH, nanobubbles, laser

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