The complete mitochondrial genome of Illiberis ulmivora (Lepidoptera: Zygaenidae) and phylogenetic analysis

Abstract

Here we sequenced and annotated the complete mitochondrial genome (mitogenome) of Illiberis ulmivora (Lepidoptera: Zygaenidae). This mitogenome was 15,334 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA unit genes (rrnL and rrnS) and a large non-coding region (putative control region). Compared with the putative ancestral arrangement of insects, gene rearrangements were found in two tRNA clusters (IQM and ARNS1EF) of the I. ulmivora mitogenome: changing trnI-trnQ-trnM to trnM-trnI-trnQ, and changing trnS1-trnE-trnF to trnE-trnS1-trnF. The I. ulmivora mitogenome with an A + T content of 81.47% presented a negative AT-skew (−0.029) and a negative GC-skew (−0.206). All tRNAs had a typical secondary cloverleaf structure, except for trnS1 which lacked the dihydrouridine arm. The maximum likelihood analysis based on mitogenomic data highly supported the monophyly of each of Zygaenidae and Limacodidae within Zygaenoidea, and I. ulmivora was sister to the other four Zygaenidae species.

Keywords

• insects
• mitochondrial dna
• zygaenoidea
• phylogenetic analysis
• gene rearrangement