Nature Communications (Feb 2020)

Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

  • Xiaoqin Zhong,
  • Hao Chen,
  • Richard N. Zare

DOI
https://doi.org/10.1038/s41467-020-14877-x
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 9

Abstract

Read online

Mass spectrometry (MS)-based protein sequencing usually relies on in-solution proteolytic digestion, which is time-consuming and inefficient for certain proteins. Here, the authors achieve full protein sequence coverage in less than 1 ms by subjecting protein-protease mixtures to electrosonic spray ionization-MS.