Nature Communications (Feb 2020)
Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
Abstract
Mass spectrometry (MS)-based protein sequencing usually relies on in-solution proteolytic digestion, which is time-consuming and inefficient for certain proteins. Here, the authors achieve full protein sequence coverage in less than 1 ms by subjecting protein-protease mixtures to electrosonic spray ionization-MS.