Multistable and dynamic CRISPRi-based synthetic circuits

Javier Santos-Moreno; Eve Tasiudi; Joerg Stelling; Yolanda Schaerli

doi:10.1038/s41467-020-16574-1

Nature Communications (Jun 2020)

Multistable and dynamic CRISPRi-based synthetic circuits

Javier Santos-Moreno,
Eve Tasiudi,
Joerg Stelling,
Yolanda Schaerli

Affiliations

Javier Santos-Moreno: Department of Fundamental Microbiology, University of Lausanne
Eve Tasiudi: Department of Biosystems Science and Engineering, ETH Zurich and SIB Swiss Institute of Bioinformatics
Joerg Stelling: Department of Biosystems Science and Engineering, ETH Zurich and SIB Swiss Institute of Bioinformatics
Yolanda Schaerli: Department of Fundamental Microbiology, University of Lausanne

DOI: https://doi.org/10.1038/s41467-020-16574-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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