Study on the effects of extraction conditions on the extraction efficiency of cordycepin from Cordycep militaris Linn. Link

Abstract

Cordyceps militaris Linn. Link is a fungus with high content of bioactive substances. Cordycepin is an important ingredient that determines the medicinal quality of Cordyceps sp. In this report, we studied the effects of solvents (type of solvents and solvent: water ratio), temperature, pH, incubation time with ultrasonication and number of extraction round on the extraction effiency of cordycepin from Cordyceps militaris fruiting bodies. Our results showed that, highest extraction efficiency achieved when using ethanol: water, 2:1 (v/v) as the extraction solvent; pH of the solvent should be at 7; the optimal incubation temperature was at 55 C. Furthermore, the samples should be ultrasonicated for 6 minutes with 2 pulses of 3 minutes, vortex for 1 min and 3 mins rest between pulses.

Keywords

• cordyceps militaris
• cordycepin
• quả thể
• tách chiết
• extraction
• fruiting bodies