Chromato-mass spectrometric study of valerian infusion with various extractants

Abstract

Valerian Officinalis (Valeriana officinalis L.s.p.) is a collective species in Ukraine. It contains 13 species, including in the south of Ukraine grows valerian (V. stolonifera Czern.). At the present stage, it is confirmed that the calming and antispasmodic properties of valerian raw materials are due to the content of valepotriates, sesquiterpenoids and aromatic substances, in particular eugenol derivatives. That is why in many countries around the world a number of drugs have been created on their basis. The aim of our research is to determine the component composition of tinctures of tinctures of V. stolonifera Czern. made of 70 % alcohol and methanol using gas chromatography. Materials and methods. Raw materials (underground organs) Valerianae radices were harvested in March 2020 in Zaporizhzhia, Kantserivska Balka. Valerian tincture was prepared from fresh raw materials according to the method of making tinctures. The tincture was investigated on a gas chromatograph Agilent 7890B with mass spectrometric detector 5977B. The NIST14 mass spectrum library was used to identify the components. Results. 77 components were identified by gas chromatography in methanolic tincture of V. stolonifera Czern. roots, 13 components were isolated quantitatively. In the analysis of the chromatogram and the characteristic of the area of the peaks of the alcoholic tincture of V. stolonifera Czern. roots, 78 components were identified, 11 components were isolated quantitatively. At the comparative characteristic of chromatograms 39 components coincide. Of the main components, the largest content is: 13.413 RT methanolic 1(2H)-Naphthalenone, octahydro-4a,8a-dimethyl-7-(1-methylethyl)-, [4aR-(4a.α.,7.β.,8a.α.)] – 5.34 % 13.414 RT alcohol – 8.41 %; 11.943 RT methanolic Myrtenyl isovalerate – 3.88 %; 11,942 RT alcohol – 5.15 %; 15.362 RT methanolic Kessanyl acetate – 2.52 %; 15.360 RT Kessanyl acetate alcohol – 1.41 %. Conclusions. It was seen that valerian tinctures with methanol and alcohol extractant differ in both quantitative and qualitative composition analyzing the obtained data of gas chromatography. 39 Compounds were present in all tinctures; three of them were present in large quantities. According to the results of the study, data have been obtained that show that the best indicators of the quantitative and qualitative composition of the tincture of V. stolonifera Czern. were obtained using the extractant 70 % alcohol.

Keywords

• valeriana stolonifera czern.
• tincture
• chromato-mass spectrometry
• 70 % alcohol
• methanol