Simulation of Increasing the Cell Membrane Permeability Under Steady-State Conditions

Abstract

There is being presented simulation of increasing membrane permeability cellular baker's yeast for the set values concentrations of alcohol solutions. It allows for easy activity forecasting intracellular catalase. Due to the costs associated with disposal large volumes of waste and the purchase of permeabilizing agents the tolerance of the permeabilization process to changes in alcohols concentration was checked. For this purpose, appropriate mathematical models were used. The obtained results confirm the high sensitivity of the process to changes in concentration permeabilizing factor. Of the three permeabilizations studied, this is the process using ethanol turned out to be the least tolerant to change concentration of the permeabilizing factor. The concentration of the solution of this alcohol, which will be used in permeabilization to obtain cells yeast with catalase activity half of the optimal activity, is only 20% lower than the optimal concentration. Processes permeabilization using the other two alcohols considered less sensitive to changes in their concentration. The concentrations of 1-propanol and 2-propanol solutions for AS = ½ Aopt are 2.5 times and 3.5 times lower than the optimal concentrations, respectively.

Keywords

• simulations
• computer modeling
• permeabilization
• catalase
• optimization
• saccharomyces cerevisiae