Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer

Abstract

Paramphistomosis is the emerging fluke infection in young ruminants which results in high morbidity and mortality in tropics and sub-tropics. The present study was carried out to detect the sharing antigens among the purified somatic antigens of G. crumenifer and C. cotylophorum, in order to use as a candidate antigen for diagnosis of paramphistomosis in sheep. Immunoblot analysis of the purified somatic antigens of C. cotylophorum and G. crumenifer using their specific rabbit hyper immune serum revealed 3 common polypeptides at the range of 27, 29 and 60 kDa. Hence these three immunodominant peptides can be used candidate antigens in development of vaccines against ovine paraphistomosis and also for early diagnosis of infection.

Keywords

• Paramphistomes
• Somatic antigen
• SDS-PAGE
• Western blot